Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media

Kotaro Kameda, Shota Someya, Jun Fujita, Keiichi Fukuda, Shugo Tohyama

研究成果: Article査読

抄録

We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).

本文言語English
論文番号101341
ジャーナルSTAR Protocols
3
2
DOI
出版ステータスPublished - 2022 6月 17
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 神経科学(全般)
  • 免疫学および微生物学(全般)
  • 医学(全般)

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