Purification and characterization of arylmalonate decarboxylase from Achromobacter sp. KU1311

Kenji Miyamoto, Yoshito Yatake, Keisuke Tamura, Yosuke Terao, Hiromichi Ohta

研究成果: Article査読

13 被引用数 (Scopus)

抄録

We have isolated, purified and characterized arylmalonate decarboxylase (AMDase; EC 4.1.1.76). This is an unique enzyme that gives optically pure arylpropionates from the corresponding arylmalonates. Recently, we have screened similar enzyme producers from soil samples and succeeded in isolating Achromobacter sp. KU1311. The gene encoding the enzyme was cloned and sequenced. The AMDase gene consists of 720 nucleotides, which specifies a 240 amino acid protein with a relative molecular mass of 24,735. This enzyme was purified and its characteristics were compared with those of the hitherto known enzyme from Alcaligenes bronchisepticus KU1201.

本文言語English
ページ(範囲)263-267
ページ数5
ジャーナルJournal of Bioscience and Bioengineering
104
4
DOI
出版ステータスPublished - 2007 10

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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