Purification of dFMR1-containing complexes using tandem affinity purification

Keita Miyoshi, Akiyo Ogino, Mikiko C. Siomi, Haruhiko Siomi

研究成果: Chapter

抄録

Fragile X syndrome results from the lack of FMR1 expression. To understand how the lack of FMR1 function leads to the syndrome, we are studying the Drosophila FMR1 related protein (dFMR1). We performed affinity purification of dFMR1-associated complexes from cultured Drosophila S2 cells and found that dFMR1 associates with a key component of RNA interference, AGO2. Our finding suggests cross talk between the fragile X syndrome protein and RNA interference. In this chapter, we describe a tandem affinity purification method to isolate the protein components and small RNAs in the dFMR1-associated complexes.

本文言語English
ホスト出版物のタイトルTrinucleotide Repeat Protocols
ページ111-121
ページ数11
DOI
出版ステータスPublished - 2013 7 24

出版物シリーズ

名前Methods in Molecular Biology
1010
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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