Purification of two transcription factors required for initiation by mammalian RNA polymerase II

Tatsuo Kawaguchi, Shigetaka Kitajima, Yoshiyuki Niho, Taiko Oda, Joseph Germino, Sherman M. Weissman, Yukio Yasukochi

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of ≈33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.

本文言語English
ページ(範囲)6619-6623
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
87
17
DOI
出版ステータスPublished - 1990 9月
外部発表はい

ASJC Scopus subject areas

  • 一般

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