Pyrrolidinium-type fullerene derivative-induced apoptosis by the generation of reactive oxygen species in HL-60 cells

Chiho Nishizawa, Noriyuki Hashimoto, Sachiko Yokoo, Megumi Funakoshi-Tago, Tadashi Kasahara, Kyoko Takahashi, Shigeo Nakamura, Tadahiko Mashino

研究成果: Article査読

35 被引用数 (Scopus)

抄録

The biological activities of C60-bis(N,N-dimethylpyrrolidinium iodide), a water-soluble cationic fullerene derivative, on human promyeloleukaemia (HL-60) cells were investigated. The pyrrolidinium fullerene derivative showed cytotoxicity in HL-60 cells. The characteristics of apoptosis, such as DNA fragmentation and condensation of chromatin in HL-60 cells, were observed by exposure to the pyrrolidinium fullerene derivative. Caspase-3 and-8 were activated and cytochrome c was also released from mitochondria. The generation of reactive oxygen species (ROS) by the pyrrolidinium fullerene derivative was observed by DCFH-DA, a fluorescence probe for the detection of ROS. Pre-treatment with α-tocopherol suppressed cell death and intracellular oxidative stress caused by the pyrrolidinium fullerene derivative. The apoptotic cell death induced by the pyrrolidinium fullerene derivative was suggested to be mediated by ROS generated by the pyrrolidinium fullerene derivative.

本文言語English
ページ(範囲)1240-1247
ページ数8
ジャーナルFree Radical Research
43
12
DOI
出版ステータスPublished - 2009

ASJC Scopus subject areas

  • 生化学

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