TY - GEN
T1 - Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors
AU - Ito, Yoshihisa
AU - Osaki, Toshihisa
AU - Kamiya, Koki
AU - Yamada, Tetsuya
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Funding Information:
This work was partly supported by KAKENHI (JP17H02758), JSPS, Strategic Advancement of MultiPurpose Ultra-Human Robot and Artificial Intelligence Technologies Project of NEDO, and the Regional Innovation Strategy Support Program of MEXT, Japan. The authors thank to Mses. Uchida and Hashino (KISTEC) for their kind assistance.
Publisher Copyright:
© 2019 IEEE.
PY - 2019/6
Y1 - 2019/6
N2 - This work designs and demonstrates the lipid bilayer-based ultra-sensitive biosensor capable of short-time detection in a quantitative manner, which is achieved by connecting multiple sensor elements in parallel.Artificial cell membranes with functional membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena.In this work, we connected independent membrane sensors in parallel, where the ionic current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. We developed a quad sensor assembling four membranes in parallel, and examined detection time of a single membrane sensor, the quad sensor, and four parallel quad-sensors. 1-Gω resistor was installed in series with each membrane to avoid overload current caused by membrane rupture. The results showed significant improvement in the detection time and deviation by the parallelization, which promises quantitative monitoring with the stochastic sensor.
AB - This work designs and demonstrates the lipid bilayer-based ultra-sensitive biosensor capable of short-time detection in a quantitative manner, which is achieved by connecting multiple sensor elements in parallel.Artificial cell membranes with functional membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena.In this work, we connected independent membrane sensors in parallel, where the ionic current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. We developed a quad sensor assembling four membranes in parallel, and examined detection time of a single membrane sensor, the quad sensor, and four parallel quad-sensors. 1-Gω resistor was installed in series with each membrane to avoid overload current caused by membrane rupture. The results showed significant improvement in the detection time and deviation by the parallelization, which promises quantitative monitoring with the stochastic sensor.
KW - Artificial cell membranes
KW - Nanopore
KW - Parallelization
KW - Rapid detection
KW - Stochastic biosensors
UR - http://www.scopus.com/inward/record.url?scp=85071908418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071908418&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2019.8808191
DO - 10.1109/TRANSDUCERS.2019.8808191
M3 - Conference contribution
AN - SCOPUS:85071908418
T3 - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
SP - 6
EP - 8
BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Y2 - 23 June 2019 through 27 June 2019
ER -