Quantitation of membrane-associated crystallins from aging and cataractous human lenses

M. Takehana, L. Takemoto

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Membrane from individual cataractous and normal human lenses has been prepared by discontinuous sucrose gradient centrifugation. A sensitive solid-phase radioimmunoassay has been developed to quantitate the levels of α, β, and γ crystallins associated with membrane obtained from each sucrose gradient interface. The results of this analysis demonstrate significant levels of all three crystallins associated with purified membrane. Although the relative percentage of each membrane-associated crystallin does not change during aging and/or cataractogenesis of the human lens, membrane from cataractous lenses is characterized by greater total amounts of crystallins associated with purified membrane obtained from denser interfaces of the discontinuous sucrose gradient.

本文言語English
ページ(範囲)780-784
ページ数5
ジャーナルInvestigative Ophthalmology and Visual Science
28
5
出版ステータスPublished - 1987 1月 1

ASJC Scopus subject areas

  • 眼科学
  • 感覚系
  • 細胞および分子神経科学

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