Quantitative evaluation of color difference between actual specimens and whole-slide imaging-scanned images calibrated with commercial color charts

Yusuke Murayama, Tohru Sugiyama, Yoshihiko Ogino, Hiroki Furuta, Takaaki Kambara, Koi Kamisono, Tokiya Abe, Katsura Emoto, Akinori Hashiguchi, Yoichi Kajimura, Michiie Sakamoto

研究成果: Conference contribution

抄録

When using different cameras and displays in the shot and display of one subject, different color images are often showed. To solve this problem, we have developed a color chart in which the constituent colors dyed with dyes are evenly distributed in the color space. We have also developed a tool that creates an International Color Consortium (ICC) profile from the captured image of this color chart. In this report, we will describe the color difference correction accuracy when our method is applied to actual stained pathological specimens taken with multiple whole-slide imaging (WSI). We confirmed color correction accuracy of Lab values in major parts such as the cell nucleus. The Lab value of the specimen itself measured by a spectrocolorimeter was compared with that of the captured image. As a result, the color difference ?E in H&E-stained cell nucleus was improved from 32.2 to 6.4 for Nanozoomer and from 13.5 to 7.2 for ultra-fast scanner (UFS) by our color correction. The results of the evaluations for other areas and other stain methods (PAS, EVG, MT, and PAM) were good. In the future, high-accuracy color correction of teacher data/evaluation data in AI diagnosis using pathological images will be important.

本文言語English
ホスト出版物のタイトルMedical Imaging 2022
ホスト出版物のサブタイトルDigital and Computational Pathology
編集者John E. Tomaszewski, Aaron D. Ward, Richard M. Levenson
出版社SPIE
ISBN(電子版)9781510649538
DOI
出版ステータスPublished - 2022
イベントMedical Imaging 2022: Digital and Computational Pathology - Virtual, Online
継続期間: 2022 3月 212022 3月 27

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
12039
ISSN(印刷版)1605-7422

Conference

ConferenceMedical Imaging 2022: Digital and Computational Pathology
CityVirtual, Online
Period22/3/2122/3/27

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 原子分子物理学および光学
  • 生体材料
  • 放射線学、核医学およびイメージング

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