Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry

Tomoyoshi Soga, Yoshiaki Ohashi, Yuki Ueno, Hisako Naraoka, Masaru Tomita, Takaaki Nishioka

研究成果: Article査読

763 被引用数 (Scopus)

抄録

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.

本文言語English
ページ(範囲)488-494
ページ数7
ジャーナルJournal of Proteome Research
2
5
DOI
出版ステータスPublished - 2003 9 1

ASJC Scopus subject areas

  • 生化学
  • 化学 (全般)

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