Rab GTPases-dependent endocytic pathways regulate neuronal migration and maturation through N-cadherin trafficking

Takeshi Kawauchi, Katsutoshi Sekine, Mima Shikanai, Kaori Chihama, Kenji Tomita, Kenichiro Kubo, Kazunori Nakajima, Yo ichi Nabeshima, Mikio Hoshino

研究成果: Article査読

231 被引用数 (Scopus)

抄録

Although membrane trafficking pathways are involved in basic cellular functions, the evolutionally expanded number of their related family proteins suggests additional roles for membrane trafficking in higher organisms. Here, we show that several Rab-dependent trafficking pathways differentially participate in neuronal migration, an essential step for the formation of the mammalian-specific six-layered brain structure. In vivo electroporation-mediated suppression of Rab5 or dynamin to block endocytosis caused a severe neuronal migration defect in mouse cerebral cortex. Among many downstream endocytic pathways, suppression of Rab11-dependent recycling pathways exhibited a similar migration disorder, whereas inhibition of Rab7-dependent lysosomal degradation pathways affected only the final phase of neuronal migration and dendrite morphology. Inhibition of Rab5 or Rab11 perturbed the trafficking of N-cadherin, whose suppression also disturbed neuronal migration. Taken together, our findings reveal physiological roles of endocytic pathways, each of which has specific functions in distinct steps of neuronal migration and maturation during mammalian brain formation.

本文言語English
ページ(範囲)588-602
ページ数15
ジャーナルNeuron
67
4
DOI
出版ステータスPublished - 2010 8月

ASJC Scopus subject areas

  • 神経科学(全般)

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