Radiation-induced chromosome aberrations in ataxia telangiectasia cells: High frequency of deletions and misrejoining detected by fluorescence in situ hybridization

Tetsuya Kawata, Hisao Ito, Kerry George, Honglu Wu, Takashi Uno, Kouichi Isobe, Francis A. Cucinotta

研究成果: Article査読

16 被引用数 (Scopus)

抄録

The mechanisms underlying the hyper-radiosensitivity of AT cells were investigated by analyzing chromosome aberrations in the G2 and M phases of the cell cycle using a combination of chemically induced premature chromosome condensation (PCC) and fluorescence in situ hybridization (FISH) with chromosome painting probes. Confluent cultures of normal fibroblast cells (AG1522) and fibroblast cells derived from an individual with AT (GM02052) were exposed to γ rays and allowed to repair at 37°C for 24 h. At doses that resulted in 10% survival, GM02052 cells were approximately five times more sensitive to γ rays than AG1522 cells. For a given dose, GM02052 cells contained a much higher frequency of deletions and misrejoining than AG1522 cells. For both cell types, a good correlation was found between the percentage of aberrant cells and cell survival. The average number of color junctions, which represent the frequency of chromosome misrejoining, was also found to correlate well with survival. However, in a similar surviving population of GM02052 and AG1522 cells, induced by 1 Gy and 6 Gy, respectively, AG1522 cells contained four times more color junctions and half as many deletions as GM02052 cells. These results indicate that both repair deficiency and misrepair may be involved in the hyper-radiosensitivity of AT cells.

本文言語English
ページ(範囲)597-603
ページ数7
ジャーナルRadiation Research
159
5
DOI
出版ステータスPublished - 2003 5 1

ASJC Scopus subject areas

  • 生物理学
  • 放射線
  • 放射線学、核医学およびイメージング

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