Radiation-sensitizing effect of low-concentration docetaxel on human esophageal squamous cell carcinoma cell lines

Satoshi Tabuchi, Soji Ozawa, Kazuo Koyanagi, Naoyuki Shigematsu, Atsushi Kubo, Masakazu Ueda, Yuko Kitagawa Kitajima, Masaki Kitajima

研究成果: Article

5 引用 (Scopus)

抄録

Esophageal squamous cell carcinoma (ESCC) is more sensitive to radiation and chemotherapy than other cancers of the digestive system, and combined modality therapy may represent a promising treatment method. The radiation-sensitizing effect of docetaxel on ESCC cell lines was investigated. A colony formation assay was performed in which ESCC cell lines (TE2, TE3) and A431 were exposed to docetaxel (from 1.0x10 11 to 107 M) for 3 h to determine the concentration of docetaxel that was not able to kill individual cells (i.e., the non-cytocidal concentration). Individual cell lines were then exposed to the non-cytocidal concentration of docetaxel prior to, during, and after irradiation to determine whether the timing of docetaxel administration affected cell survival. In addition, flow-cytometry was performed, and the cell cycle was examined prior to and after docetaxel exposure to assess the mechanism of docetaxel as a radiation sensitizer. Docetaxel exhibited a concentration-dependent cytocidal effect, with a different IC50 for each cell type. Almost no cytocidal effect was observed at the following docetaxel concentrations: A431, ≤1.0x1010 M; TE-2 and TE-3, ≤1.0x109 M. Concurrent treatment with docetaxel and radiation tended to decrease cell survival in all the cell lines compared with docetaxel or radiation alone. Cell survival was lowest when the cells were treated using X-ray irradiation after docetaxel exposure (p<0.05). Flow cytometry revealed that in all three cell lines, docetaxel exposure increased the G2/M cell fraction with a higher increase in the cell line that exhibited the highest radiosensitivity This study demonstrated that the administration of docetaxel at a non-cytocidal concentration prior to radiotherapy produced a synergistic cell-killing effect in SCC cell lines.

元の言語English
ページ(範囲)601-606
ページ数6
ジャーナルExperimental and Therapeutic Medicine
2
発行部数4
DOI
出版物ステータスPublished - 2011 7

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docetaxel
Radiation-Sensitizing Agents
Cell Line
Cell Survival
Radiation
Esophageal Squamous Cell Carcinoma
Flow Cytometry
Digestive System Neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • Cancer Research
  • Immunology and Microbiology (miscellaneous)

これを引用

Radiation-sensitizing effect of low-concentration docetaxel on human esophageal squamous cell carcinoma cell lines. / Tabuchi, Satoshi; Ozawa, Soji; Koyanagi, Kazuo; Shigematsu, Naoyuki; Kubo, Atsushi; Ueda, Masakazu; Kitajima, Yuko Kitagawa; Kitajima, Masaki.

:: Experimental and Therapeutic Medicine, 巻 2, 番号 4, 07.2011, p. 601-606.

研究成果: Article

Tabuchi, Satoshi ; Ozawa, Soji ; Koyanagi, Kazuo ; Shigematsu, Naoyuki ; Kubo, Atsushi ; Ueda, Masakazu ; Kitajima, Yuko Kitagawa ; Kitajima, Masaki. / Radiation-sensitizing effect of low-concentration docetaxel on human esophageal squamous cell carcinoma cell lines. :: Experimental and Therapeutic Medicine. 2011 ; 巻 2, 番号 4. pp. 601-606.
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