Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain

Hiroshi Itoh, Kazuwa Nakao, Yoshihiko Saito, Takayuki Yamada, Gotaro Shirakami, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Shin ichi Suga, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Hiroo Imura

研究成果: Article

47 引用 (Scopus)

抄録

We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.

元の言語English
ページ(範囲)120-128
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
158
発行部数1
DOI
出版物ステータスPublished - 1989 1 16
外部発表Yes

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Brain Natriuretic Peptide
Radioimmunoassay
Canidae
Brain
Swine
Medulla Oblongata
Olfactory Bulb
Gel permeation chromatography
Atrial Natriuretic Factor
Mesencephalon
Haplorhini
Gel Chromatography
Rats
Molecular Weight
Molecular weight

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain. / Itoh, Hiroshi; Nakao, Kazuwa; Saito, Yoshihiko; Yamada, Takayuki; Shirakami, Gotaro; Mukoyama, Masashi; Arai, Hiroshi; Hosoda, Kiminori; Suga, Shin ichi; Minamino, Naoto; Kangawa, Kenji; Matsuo, Hisayuki; Imura, Hiroo.

:: Biochemical and Biophysical Research Communications, 巻 158, 番号 1, 16.01.1989, p. 120-128.

研究成果: Article

Itoh, H, Nakao, K, Saito, Y, Yamada, T, Shirakami, G, Mukoyama, M, Arai, H, Hosoda, K, Suga, SI, Minamino, N, Kangawa, K, Matsuo, H & Imura, H 1989, 'Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain', Biochemical and Biophysical Research Communications, 巻. 158, 番号 1, pp. 120-128. https://doi.org/10.1016/S0006-291X(89)80186-X
Itoh, Hiroshi ; Nakao, Kazuwa ; Saito, Yoshihiko ; Yamada, Takayuki ; Shirakami, Gotaro ; Mukoyama, Masashi ; Arai, Hiroshi ; Hosoda, Kiminori ; Suga, Shin ichi ; Minamino, Naoto ; Kangawa, Kenji ; Matsuo, Hisayuki ; Imura, Hiroo. / Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain. :: Biochemical and Biophysical Research Communications. 1989 ; 巻 158, 番号 1. pp. 120-128.
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AU - Itoh, Hiroshi

AU - Nakao, Kazuwa

AU - Saito, Yoshihiko

AU - Yamada, Takayuki

AU - Shirakami, Gotaro

AU - Mukoyama, Masashi

AU - Arai, Hiroshi

AU - Hosoda, Kiminori

AU - Suga, Shin ichi

AU - Minamino, Naoto

AU - Kangawa, Kenji

AU - Matsuo, Hisayuki

AU - Imura, Hiroo

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