Rapid and accurate IC50 determination using logarithmic concentration generator

Yuta Abe, Hirotaka Sasaki, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Norihisa Miki, Shoji Takeuchi

研究成果: Conference contribution

抄録

This paper proposes an effective methodology for the determination of the half maximal (50%) inhibitory concentration (IC50) by using microfluidic device that generates logarithmic concentration gradients. Dose-response curve is obtained with respect to the concentrations in a logarithmic scale. Although we have presented a high-throughput analysis of ATP binding cassette transporter (ABC-transporter) by using linear concentration generator [1] and fluorescent substrates [2], logarithmic concentration generator is demanded for rapid and accurate IC50 determination as shown in Fig. 1. We designed a microfluidic device composed of branching micro-channels where samples are divided and mixed to generate designated concentrations. Finally inhibition analysis of substrate transport was conducted by linear and logarithmic concentration generators. We found that logarithmic concentration generators are useful for the accurate determination of IC50 by a fewer number of tests than linear concentration generators.

本文言語English
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版社Chemical and Biological Microsystems Society
ページ956-958
ページ数3
ISBN(印刷版)9780979806452
出版ステータスPublished - 2012
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
継続期間: 2012 10 282012 11 1

出版物シリーズ

名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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