Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

Sravan Kumar Goparaju, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yuki Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru Ko, Minoru Ko

研究成果: Article

17 引用 (Scopus)

抄録

Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.

元の言語English
記事番号42367
ジャーナルScientific Reports
7
DOI
出版物ステータスPublished - 2017 2 13

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Pluripotent Stem Cells
Transcription Factors
Neurons
Messenger RNA
Preclinical Drug Evaluations
Regenerative Medicine
Electrophysiology
Cell Transplantation
Motor Neurons
Cell- and Tissue-Based Therapy
Calcium
Phenotype

ASJC Scopus subject areas

  • General

これを引用

Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. / Goparaju, Sravan Kumar; Kohda, Kazuhisa; Ibata, Keiji; Soma, Atsumi; Nakatake, Yuki; Akiyama, Tomohiko; Wakabayashi, Shunichi; Matsushita, Misako; Sakota, Miki; Kimura, Hiromi; Yuzaki, Michisuke; Ko, Shigeru; Ko, Minoru.

:: Scientific Reports, 巻 7, 42367, 13.02.2017.

研究成果: Article

Goparaju, Sravan Kumar ; Kohda, Kazuhisa ; Ibata, Keiji ; Soma, Atsumi ; Nakatake, Yuki ; Akiyama, Tomohiko ; Wakabayashi, Shunichi ; Matsushita, Misako ; Sakota, Miki ; Kimura, Hiromi ; Yuzaki, Michisuke ; Ko, Shigeru ; Ko, Minoru. / Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. :: Scientific Reports. 2017 ; 巻 7.
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AU - Akiyama, Tomohiko

AU - Wakabayashi, Shunichi

AU - Matsushita, Misako

AU - Sakota, Miki

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