Rapid formation of size-controlled 3-dimensional hetero-spheroid using micro-rotational flow

T. Kodama, H. Ota, N. Miki

研究成果: Conference contribution

抄録

In this paper, we demonstrate formation of size-controlled 3-dimensional hetero-spheroids using a micro-fluidic device in approximately 2 min. The device collects cells in the center of a micro-chamber using micro-rotational flow as shown in Fig. 1. In previous work, we used this device to generate size-controlled HepG2 spheroids [1]. We applied it to form hetero-spheroids consisting of HepG2 and HUVEC. We experimentally found that the spheroid sizes could be controlled by the cell densities of the media independent of the ratio of HepG2 and HUVEC. Uniform dispersion of HepG2 and HUVEC in the formed spheroid was also experimentally verified.

本文言語English
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ359-361
ページ数3
出版ステータスPublished - 2011 12 1
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
継続期間: 2011 10 22011 10 6

出版物シリーズ

名前15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
国/地域United States
CitySeattle, WA
Period11/10/211/10/6

ASJC Scopus subject areas

  • 制御およびシステム工学

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