Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

研究成果: Conference contribution

抄録

This paper describes measurement system of membrane proteins with a rapid perfusion capability using microfluidic channels. In the previous report, we successfully recorded channel conductance of alpha-hemolysine (αHL) in bilayer lipid membranes (BLMs) using droplets contacting method with PDWC (parylene double well chip). This method is one of the easiest ways for preparing the BLMs, just pipetting droplets of the solution. The drawback of the droplet-based systems is the incapability of exchanging solution. To efficiently test the effect of inhibitors and promoters of membrane proteins in drug discovery, the solution exchange is mandatory. In this study, we proposed the concept of solution exchange in the system using microfluidic channel. We demonstrated rapid perfusion that is capable of exchanging the solution within 20 s in this droplets contacting system. We experimentally evaluated the system in terms of the flow rate and the BLM stability and demonstrated the binding assay of a membrane protein using its blockers. We believe that this method is necessary for efficient drug screening in our droplet-based system.

本文言語English
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版社Chemical and Biological Microsystems Society
ページ683-685
ページ数3
ISBN(印刷版)9780979806452
出版ステータスPublished - 2012
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
継続期間: 2012 10 282012 11 1

出版物シリーズ

名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
国/地域Japan
CityOkinawa
Period12/10/2812/11/1

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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