Rat Kupffer cell-derived nitric oxide modulates induction of lymphokine-activated killer cell

Iwao Kurose, Soichiro Miura, Hidetsugu Saito, Shinichiro Tada, Dai Fukumura, Hajime Higuchi, Hiromasa Ishii

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Background & Aims: Nitric oxide is now recognized to regulate immune responses and cell viability in various organs. The present study was designed to clarify whether NO released from Kupffer cells modulates the lymphokine-activated killer (LAK) activity of interleukin 2 (IL-2)-treated splenocytes. Methods: Splenocytes and Kupffer cells were isolated from male Wistar rats and cocultured for 48 hours in the presence of lipopolysaccharide (1 μg/mL). The splenocyte LAK activity and expression of IL-2 receptor were determined. Results: Kupffer cells with lipopolysaccharide reduced the IL-2 receptor expression and LAK activity of splenocytes. The addition of either NG-monomethyl-l-arginine, an inhibitor of NO synthesis, or aminoguanidine, an inhibitor of inducible NO synthase, to the medium reversed the suppression of IL-2 receptor expression and LAK activity by lipopolysaccharide-stimulated Kupffer cells. 8-bromoguanosine 3′,5′-cyclic monophosphate and NO donors decreased the splenocyte LAK activity and IL-2 receptor expression. Treatment with lipopolysaccharide increased the inducible NO synthase activity as well as the nitrite and nitrate levels in the culture medium of Kupffer cells but not in splenocytes. Conclusions: The results of this study suggest that NO produced by the inducible NO synthase of Kupffer cells in response to lipopolysaccharide modulates the IL-2 receptor expression and LAK activity of splenocytes.

本文言語English
ページ(範囲)1958-1968
ページ数11
ジャーナルGastroenterology
109
6
DOI
出版ステータスPublished - 1995 12月
外部発表はい

ASJC Scopus subject areas

  • 肝臓学
  • 消化器病学

フィンガープリント

「Rat Kupffer cell-derived nitric oxide modulates induction of lymphokine-activated killer cell」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル