OCl-/HOCl imposed a rapid inactivation of catalase (hydrogen-peroxide: hydrogen-peroxide oxidoreductase, EC 22.214.171.124), some of which was slowly reversible upon subsequent exposure to H2O2. Ethanol accelerated this restoration of activity by H2O2. OCl- caused biphasic changes in the visible absorption spectrum of catalase, which were partially reversed by dithionite. A scheme of reactions involving axial ligation of one or two OCl- to heme iron, followed by heterolytic or homolytic cleavages of the OCl bond, is proposed to account for the behavior of the system.
|ジャーナル||Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular|
|出版ステータス||Published - 1988 8月 31|
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