Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F-induced luteolysis in rats

Kazuhiro Minegishi, Mamoru Tanaka, Osamu Nishimura, Shinji Tanigaki, Kei Miyakoshi, Hitoshi Ishimoto, Yasunori Yoshimura

研究成果: Article査読

26 被引用数 (Scopus)


We investigated the contribution of neutrophils to prostaglandin (PG)F-induced luteolysis and the role of reactive oxygen species (ROS) as potential mediators of neutrophil accumulation in regressing corpora lutea of superovulated rats. On day 8 of pseudopregnancy, subcutaneous injection of PGF (500 μg) significantly increased rhodamine 6G-labeled leukocyte adhesion in luteal venules, as observed by intravital microscopy. Neutrophil accumulation was confirmed by significantly increased myeloperoxidase (MPO) activity. Pretreatment with anti-leukocyte antibody (CD18-directed monoclonal antibody, WT-3) significantly inhibited the PGF-induced increases in adherent leukocytes and MPO activity. Anti-leukocyte antibody also maintained serum progesterone concentrations. Pretreatment with oxygen free radical scavengers, superoxide dismutase (50,000 U/kg) and catalase (90,000 U/kg), also attenuated these PGF-induced alterations. Corpora lutea preloaded with dichlorodihydrofluorescein diacetate succinimidyl ester, a fluorescent indicator for determining intracellular ROS generation, exhibited an increase in fluorescence after PGF treatment. These findings suggest that leukocyte-endothelium interactions mediated by ROS generation are important in PGF-induced luteolysis in rats.

ジャーナルAmerican Journal of Physiology - Endocrinology and Metabolism
6 46-6
出版ステータスPublished - 2002 12 1

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 生理学
  • 生理学(医学)


「Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F<sub>2α</sub>-induced luteolysis in rats」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。