Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168

K. Tsuge, M. Itaya

研究成果: Article

27 引用 (Scopus)

抄録

Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the θ-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants of B. subtilis. In contrast, linearized pGETS103 gave tetracycline-resistant transformants only when the recipient strain had the pBR322 sequence in the genome. The efficiency and fidelity of the recombinational transfer of DNAs of up to 90 kb are demonstrated.

元の言語English
ページ(範囲)5453-5458
ページ数6
ジャーナルJournal of Bacteriology
183
発行部数18
DOI
出版物ステータスPublished - 2001
外部発表Yes

Fingerprint

Bacillus subtilis
Plasmids
DNA
Tetracycline
Genome
Sequence Homology
Bacillus
Escherichia coli

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

これを引用

Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168. / Tsuge, K.; Itaya, M.

:: Journal of Bacteriology, 巻 183, 番号 18, 2001, p. 5453-5458.

研究成果: Article

Tsuge, K. ; Itaya, M. / Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168. :: Journal of Bacteriology. 2001 ; 巻 183, 番号 18. pp. 5453-5458.
@article{003150fd8a2b42e49777a2def3e9e137,
title = "Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168",
abstract = "Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the θ-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants of B. subtilis. In contrast, linearized pGETS103 gave tetracycline-resistant transformants only when the recipient strain had the pBR322 sequence in the genome. The efficiency and fidelity of the recombinational transfer of DNAs of up to 90 kb are demonstrated.",
author = "K. Tsuge and M. Itaya",
year = "2001",
doi = "10.1128/JB.183.18.5453-5458.2001",
language = "English",
volume = "183",
pages = "5453--5458",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "18",

}

TY - JOUR

T1 - Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168

AU - Tsuge, K.

AU - Itaya, M.

PY - 2001

Y1 - 2001

N2 - Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the θ-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants of B. subtilis. In contrast, linearized pGETS103 gave tetracycline-resistant transformants only when the recipient strain had the pBR322 sequence in the genome. The efficiency and fidelity of the recombinational transfer of DNAs of up to 90 kb are demonstrated.

AB - Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the θ-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants of B. subtilis. In contrast, linearized pGETS103 gave tetracycline-resistant transformants only when the recipient strain had the pBR322 sequence in the genome. The efficiency and fidelity of the recombinational transfer of DNAs of up to 90 kb are demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=0034873598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034873598&partnerID=8YFLogxK

U2 - 10.1128/JB.183.18.5453-5458.2001

DO - 10.1128/JB.183.18.5453-5458.2001

M3 - Article

C2 - 11514534

AN - SCOPUS:0034873598

VL - 183

SP - 5453

EP - 5458

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 18

ER -