Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168

K. Tsuge, M. Itaya

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the θ-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants of B. subtilis. In contrast, linearized pGETS103 gave tetracycline-resistant transformants only when the recipient strain had the pBR322 sequence in the genome. The efficiency and fidelity of the recombinational transfer of DNAs of up to 90 kb are demonstrated.

本文言語English
ページ(範囲)5453-5458
ページ数6
ジャーナルJournal of Bacteriology
183
18
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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