TY - JOUR
T1 - Reconstitution of immune systems in RAG2(-/-) mice by transfer with interleukin-12-induced splenic hematopoietic progenitor cells
AU - Yahata, Takashi
AU - Ando, Kiyoshi
AU - Watanabe, Kazuhito
AU - Mori, Takehiko
AU - Ohta, Akio
AU - Ohmi, Yasushi
AU - Iwakabe, Kenji
AU - Kuge, Soichi
AU - Nakui, Minoru
AU - Ito, Mamoru
AU - Habu, Sonoko
AU - Yasuda, Shuhei
AU - Nishimura, Takashi
N1 - Funding Information:
The authors would like to thank Drs Michiko Kobayashi and Steven H. Herrmann for their kind gift of IL-12. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education Science and Culture, Japan, a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare, CREST (Core Research for Environmental Science and Technology) of JST (Japan Science and Technology Corporation) and a Grant-in-Aid for the IL-12 project of Tokai University School of Medicine.
PY - 1998/7
Y1 - 1998/7
N2 - The administration of a high dose of IL-12 into the mice resulted in the induction of splenomegaly. From the flow cytometry analysis of cellularity in an enlarged spleen, it was demonstrated that Thy1.2-CD45RB-c-Kit+Sca- 1+Lin- hematopoietic progenitor cells markedly increased in IL-12- administered mouse spleen compared with untreated mouse spleen. The IL-12- induced hematopoietic progenitor cells showed a greatly enhanced colony- forming activity in CFU-granulocyte/macrophage (CFU-GM), blast-forming units- erythroid (BFU-E) and CFU-spleen (CFU-S) assay. Moreover, it was initially demonstrated that the transfer of IL-12-induced splenic hematopoietic progenitor cells into immunodeficient RAG2(-/-) mice caused a complete reconstitution of their immune functions including T- and B-cell-mediated immunity. Thus, the evidence that IL-12 has a capability of inducing hematopoietic progenitor cells possessing stem cell-like activity in vivo, indicated another important immunomodulating activity of IL-12 in immunotherapy.
AB - The administration of a high dose of IL-12 into the mice resulted in the induction of splenomegaly. From the flow cytometry analysis of cellularity in an enlarged spleen, it was demonstrated that Thy1.2-CD45RB-c-Kit+Sca- 1+Lin- hematopoietic progenitor cells markedly increased in IL-12- administered mouse spleen compared with untreated mouse spleen. The IL-12- induced hematopoietic progenitor cells showed a greatly enhanced colony- forming activity in CFU-granulocyte/macrophage (CFU-GM), blast-forming units- erythroid (BFU-E) and CFU-spleen (CFU-S) assay. Moreover, it was initially demonstrated that the transfer of IL-12-induced splenic hematopoietic progenitor cells into immunodeficient RAG2(-/-) mice caused a complete reconstitution of their immune functions including T- and B-cell-mediated immunity. Thus, the evidence that IL-12 has a capability of inducing hematopoietic progenitor cells possessing stem cell-like activity in vivo, indicated another important immunomodulating activity of IL-12 in immunotherapy.
KW - Colony-forming activity
KW - Hematopoietic progenitor, RAG2(-/-) mice
KW - IL-12
UR - http://www.scopus.com/inward/record.url?scp=0032128235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032128235&partnerID=8YFLogxK
U2 - 10.1016/S0165-2478(98)00044-3
DO - 10.1016/S0165-2478(98)00044-3
M3 - Article
C2 - 9698115
AN - SCOPUS:0032128235
SN - 0165-2478
VL - 62
SP - 165
EP - 170
JO - Immunology Letters
JF - Immunology Letters
IS - 3
ER -