Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality

Junichi Hasegawa, Atsushi Yanaihara, Shinji Iwasaki, Yukiko Otsuka, Momoko Negishi, Tomoko Akahane, Takashi Okai

研究成果: Article

36 引用 (Scopus)

抄録

Background: It has been reported that the progesterone receptor (PR) level is transiently increased within the follicle by LH stimulation and controls cumulus cells in follicles and oocyte maturation. The purpose of this study was to predict developmental competence of human oocytes during IVF via analysis of PR in cumulus cells surrounding mature oocytes. Methods: Prior to oocyte retrieval, the follicular diameter was measured and follicular fluid was collected from each mature follicle. Cumulus cells were manually separated from the oocyte-cumulus complex under a microscope. PR and PR mRNA were assessed by immunohistochemistry and real-time quantitative polymerase chain reaction (RT-PCR) measurement in human cumulus cells. Results: Immunoreactive PR-A was mainly localized in the cytoplasm and PR-B was localized in the nuclei. There was no significant relationship between PR expression and follicular diameter, follicular fluid concentration of steroids, or LH. There was no significant relationship between expression of PRs and fertilization or cleavage rate. However, PR expression was lower in the good morphology group (blastomeres ≥7 cells with fragmentation ≥5% on day 3) when compared to the other groups (P<0.05). Conclusions: These results suggest that follicular LH or steroids do not affect PR expression, and full reduction of total PR expression on cumulus cells at the time of oocyte collection is associated with good morphology in human oocytes.

元の言語English
ページ(範囲)2194-2200
ページ数7
ジャーナルHuman Reproduction
20
発行部数8
DOI
出版物ステータスPublished - 2005 8 1
外部発表Yes

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Cumulus Cells
Oocyte Retrieval
Progesterone Receptors
Embryonic Structures
Oocytes
Follicular Fluid
Steroids
Blastomeres
Fertilization
Mental Competency
Real-Time Polymerase Chain Reaction
Cytoplasm
Immunohistochemistry
Messenger RNA

ASJC Scopus subject areas

  • Reproductive Medicine
  • Rehabilitation
  • Obstetrics and Gynaecology

これを引用

Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality. / Hasegawa, Junichi; Yanaihara, Atsushi; Iwasaki, Shinji; Otsuka, Yukiko; Negishi, Momoko; Akahane, Tomoko; Okai, Takashi.

:: Human Reproduction, 巻 20, 番号 8, 01.08.2005, p. 2194-2200.

研究成果: Article

Hasegawa, Junichi ; Yanaihara, Atsushi ; Iwasaki, Shinji ; Otsuka, Yukiko ; Negishi, Momoko ; Akahane, Tomoko ; Okai, Takashi. / Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality. :: Human Reproduction. 2005 ; 巻 20, 番号 8. pp. 2194-2200.
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abstract = "Background: It has been reported that the progesterone receptor (PR) level is transiently increased within the follicle by LH stimulation and controls cumulus cells in follicles and oocyte maturation. The purpose of this study was to predict developmental competence of human oocytes during IVF via analysis of PR in cumulus cells surrounding mature oocytes. Methods: Prior to oocyte retrieval, the follicular diameter was measured and follicular fluid was collected from each mature follicle. Cumulus cells were manually separated from the oocyte-cumulus complex under a microscope. PR and PR mRNA were assessed by immunohistochemistry and real-time quantitative polymerase chain reaction (RT-PCR) measurement in human cumulus cells. Results: Immunoreactive PR-A was mainly localized in the cytoplasm and PR-B was localized in the nuclei. There was no significant relationship between PR expression and follicular diameter, follicular fluid concentration of steroids, or LH. There was no significant relationship between expression of PRs and fertilization or cleavage rate. However, PR expression was lower in the good morphology group (blastomeres ≥7 cells with fragmentation ≥5{\%} on day 3) when compared to the other groups (P<0.05). Conclusions: These results suggest that follicular LH or steroids do not affect PR expression, and full reduction of total PR expression on cumulus cells at the time of oocyte collection is associated with good morphology in human oocytes.",
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T1 - Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality

AU - Hasegawa, Junichi

AU - Yanaihara, Atsushi

AU - Iwasaki, Shinji

AU - Otsuka, Yukiko

AU - Negishi, Momoko

AU - Akahane, Tomoko

AU - Okai, Takashi

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AB - Background: It has been reported that the progesterone receptor (PR) level is transiently increased within the follicle by LH stimulation and controls cumulus cells in follicles and oocyte maturation. The purpose of this study was to predict developmental competence of human oocytes during IVF via analysis of PR in cumulus cells surrounding mature oocytes. Methods: Prior to oocyte retrieval, the follicular diameter was measured and follicular fluid was collected from each mature follicle. Cumulus cells were manually separated from the oocyte-cumulus complex under a microscope. PR and PR mRNA were assessed by immunohistochemistry and real-time quantitative polymerase chain reaction (RT-PCR) measurement in human cumulus cells. Results: Immunoreactive PR-A was mainly localized in the cytoplasm and PR-B was localized in the nuclei. There was no significant relationship between PR expression and follicular diameter, follicular fluid concentration of steroids, or LH. There was no significant relationship between expression of PRs and fertilization or cleavage rate. However, PR expression was lower in the good morphology group (blastomeres ≥7 cells with fragmentation ≥5% on day 3) when compared to the other groups (P<0.05). Conclusions: These results suggest that follicular LH or steroids do not affect PR expression, and full reduction of total PR expression on cumulus cells at the time of oocyte collection is associated with good morphology in human oocytes.

KW - Cumulus cells

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