Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome

Satoshi Tomita, Kenji Tsuge, Yo Kikuchi, Mitsuhiro Itaya

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.

本文言語English
ページ(範囲)1382-1384
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
68
6
DOI
出版ステータスPublished - 2004 6

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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