Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation

Kei Otani, Yuki Niwa, Takehiro Suzuki, Natsumi Sato, Yukiko Sasazawa, Naoshi Dohmae, Siro Simizu

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling.

本文言語English
ページ(範囲)466-472
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
498
3
DOI
出版ステータスPublished - 2018 4月 6

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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