TY - GEN
T1 - RELIABLE RE-FORMATION OF A LIPID BILAYER USING A GEOMETRICALLY GUIDED AIR BUBBLE
AU - Hashimoto, Izumi
AU - Osaki, Toshihisa
AU - Sugiura, Hirotaka
AU - Mimura, Hisatoshi
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Funding Information:
This work was partly supported by JST START Grant Number JPMJST1811, and by the Program for Building Regional Innovation Ecosystem of MEXT, Japan.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - This paper reports successful re-formation of a lipid bilayer using an air bubble. Lipid bilayers have been used for membrane protein studies and biosensor applications. Droplet-contact-method is a simple and effective method to form the bilayers. A major challenge in using the bilayers originates from their vulnerability against physical and electrical disturbance, which leads the bilayer rapture and the droplet fusion. We previously proposed a concept to re-form a bilayer by introducing an air bubble to cut-and-split the merged droplet. In this work, we investigated the key parameters to guide the bubble and drastically enhanced the yield up to 97%.
AB - This paper reports successful re-formation of a lipid bilayer using an air bubble. Lipid bilayers have been used for membrane protein studies and biosensor applications. Droplet-contact-method is a simple and effective method to form the bilayers. A major challenge in using the bilayers originates from their vulnerability against physical and electrical disturbance, which leads the bilayer rapture and the droplet fusion. We previously proposed a concept to re-form a bilayer by introducing an air bubble to cut-and-split the merged droplet. In this work, we investigated the key parameters to guide the bubble and drastically enhanced the yield up to 97%.
KW - Air bubble
KW - Lipid bilayer membrane
KW - Re-formation
KW - Robustness
UR - http://www.scopus.com/inward/record.url?scp=85136916836&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:85136916836
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 351
EP - 352
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -