Remarkable synergies between baicalein and tetracycline, and baicalein and β-lactams against methicillin-resistant Staphylococcus aureus

Mai Fujita, Sumiko Shiota, Teruo Kuroda, Tsutomu Hatano, Takashi Yoshida, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article査読

92 被引用数 (Scopus)

抄録

During the screening of compounds that potentiate the effect of antimicrobial agents against methicillin-resistant Staphylococcus aureus (MRSA), we found that an extract of thyme (Thymus vulgaris L) leaves greatly reduced the minimum inhibitory concentration (MIC) of tetracycline against MRSA. We isolated the effective compound and identified it as baicalein (5, 6, 7-trihydroxyflavone). One of the clinically isolated MRSA strains possessed tetK, a gene encoding active efflux pump for tetracycline. We examined the effect of baicalein on the efflux of tetracycline, using Escherichia coli KAM32/pTZ1252 carrying the tetK. The E. coli KAM32/pTZ1252 showed 8 to 16 times higher MIC than E. coli KAM32. We observed strong inhibition of transport of tetracycline by baicalein with membrane vesicles prepared from E. coli KAM32/pTZ1252. Baicalein also showed synergy with tetracycline in a MRSA strain that doesn't possess tetK, or with β-lactams. Thus, mechanisms of the synergies seem to be versatile.

本文言語English
ページ(範囲)391-396
ページ数6
ジャーナルMicrobiology and Immunology
49
4
DOI
出版ステータスPublished - 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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