Remarkable synergies between baicalein and tetracycline, and baicalein and β-lactams against methicillin-resistant Staphylococcus aureus

Mai Fujita, Sumiko Shiota, Teruo Kuroda, Tsutomu Hatano, Takashi Yoshida, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article

70 引用 (Scopus)

抄録

During the screening of compounds that potentiate the effect of antimicrobial agents against methicillin-resistant Staphylococcus aureus (MRSA), we found that an extract of thyme (Thymus vulgaris L) leaves greatly reduced the minimum inhibitory concentration (MIC) of tetracycline against MRSA. We isolated the effective compound and identified it as baicalein (5, 6, 7-trihydroxyflavone). One of the clinically isolated MRSA strains possessed tetK, a gene encoding active efflux pump for tetracycline. We examined the effect of baicalein on the efflux of tetracycline, using Escherichia coli KAM32/pTZ1252 carrying the tetK. The E. coli KAM32/pTZ1252 showed 8 to 16 times higher MIC than E. coli KAM32. We observed strong inhibition of transport of tetracycline by baicalein with membrane vesicles prepared from E. coli KAM32/pTZ1252. Baicalein also showed synergy with tetracycline in a MRSA strain that doesn't possess tetK, or with β-lactams. Thus, mechanisms of the synergies seem to be versatile.

元の言語English
ページ(範囲)391-396
ページ数6
ジャーナルMicrobiology and Immunology
49
発行部数4
出版物ステータスPublished - 2005
外部発表Yes

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Lactams
Methicillin-Resistant Staphylococcus aureus
Tetracycline
Thymus Plant
Escherichia coli
Microbial Sensitivity Tests
Anti-Infective Agents
baicalein
Membranes
Genes

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

これを引用

Fujita, M., Shiota, S., Kuroda, T., Hatano, T., Yoshida, T., Mizushima, T., & Tsuchiya, T. (2005). Remarkable synergies between baicalein and tetracycline, and baicalein and β-lactams against methicillin-resistant Staphylococcus aureus. Microbiology and Immunology, 49(4), 391-396.

Remarkable synergies between baicalein and tetracycline, and baicalein and β-lactams against methicillin-resistant Staphylococcus aureus. / Fujita, Mai; Shiota, Sumiko; Kuroda, Teruo; Hatano, Tsutomu; Yoshida, Takashi; Mizushima, Tohru; Tsuchiya, Tomofusa.

:: Microbiology and Immunology, 巻 49, 番号 4, 2005, p. 391-396.

研究成果: Article

Fujita, M, Shiota, S, Kuroda, T, Hatano, T, Yoshida, T, Mizushima, T & Tsuchiya, T 2005, 'Remarkable synergies between baicalein and tetracycline, and baicalein and β-lactams against methicillin-resistant Staphylococcus aureus', Microbiology and Immunology, 巻. 49, 番号 4, pp. 391-396.
Fujita, Mai ; Shiota, Sumiko ; Kuroda, Teruo ; Hatano, Tsutomu ; Yoshida, Takashi ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Remarkable synergies between baicalein and tetracycline, and baicalein and β-lactams against methicillin-resistant Staphylococcus aureus. :: Microbiology and Immunology. 2005 ; 巻 49, 番号 4. pp. 391-396.
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AU - Hatano, Tsutomu

AU - Yoshida, Takashi

AU - Mizushima, Tohru

AU - Tsuchiya, Tomofusa

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