@article{647ccfe3f1394d25a29fd8ef4b6fe0b4,
title = "Reshuffling of the Bacillus subtilis 168 genome by multifold inversion",
abstract = "The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.",
keywords = "Antibiotic resistance, Competence, Homologous recombination, Inversion, Transformation, Translocation",
author = "Azusa Kuroki and Tsutomu Toda and Kuniko Matsui and Rie Uotsu-Tomita and Masaru Tomita and Mitsuhiro Itaya",
note = "Funding Information: We thank to Drs K. Tsuge (Keio University, Yamagata, Japan) and Y. Naito (Keio University, Kanagawa, Japan) for useful discussions. This work was supported by Grant-in-Aid for the 21st Century Center of Excellence (COE) Program, entitled {\textquoteleft}{\textquoteleft}Understanding and Control of Life{\textquoteright}s Function via Systems Biology{\textquoteright}{\textquoteright} (to Keio University, Japan).",
year = "2008",
month = jan,
doi = "10.1093/jb/mvm197",
language = "English",
volume = "143",
pages = "97--105",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "1",
}