抄録
The pathogenesis of allergic conjunctivitis is largely conjectural. We investigated the possible involvement of respiratory syncytial virus (RSV), a ubiquitous respiratory pathogen, in the development of allergic conjunctivitis through immune mechanisms. A new technique of brush cytology was used to obtain conjunctival cells from 30 patients with allergic conjunctivitis and 20 control subjects. Samples were assayed for the presence of RSV sequences with the reverse-transcription polymerase chain reaction and the nested polymerase chain reaction. Specific primers and inner primers were synthesized on the basis of RNA sequences previously identified. RSV sequences were detected in 7 of 30 (23%) patient samples and 1 of 20 (5%) control samples. Our results proved that polymerase chain reaction could detect RSV sequences in conjunctival samples. RSV may be a significant pathogenic factor in allergic conjunctivitis. (J ALLERGY CLIN IMMUNOL 1995;95:663-7.).
| 元の言語 | English |
|---|---|
| ページ（範囲） | 663-667 |
| ページ数 | 5 |
| ジャーナル | The Journal of Allergy and Clinical Immunology |
| 巻 | 95 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | Published - 1995 |
| 外部発表 | Yes |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
これを引用
Respiratory syncytial virus and allergic conjunctivitis. / Fujishima, Hiroshi; Okamoto, Yoshitaka; Saito, Ichiro; Tsubota, Kazuo.
：: The Journal of Allergy and Clinical Immunology, 巻 95, 番号 3, 1995, p. 663-667.研究成果: Article
}
TY - JOUR
T1 - Respiratory syncytial virus and allergic conjunctivitis
AU - Fujishima, Hiroshi
AU - Okamoto, Yoshitaka
AU - Saito, Ichiro
AU - Tsubota, Kazuo
PY - 1995
Y1 - 1995
N2 - The pathogenesis of allergic conjunctivitis is largely conjectural. We investigated the possible involvement of respiratory syncytial virus (RSV), a ubiquitous respiratory pathogen, in the development of allergic conjunctivitis through immune mechanisms. A new technique of brush cytology was used to obtain conjunctival cells from 30 patients with allergic conjunctivitis and 20 control subjects. Samples were assayed for the presence of RSV sequences with the reverse-transcription polymerase chain reaction and the nested polymerase chain reaction. Specific primers and inner primers were synthesized on the basis of RNA sequences previously identified. RSV sequences were detected in 7 of 30 (23%) patient samples and 1 of 20 (5%) control samples. Our results proved that polymerase chain reaction could detect RSV sequences in conjunctival samples. RSV may be a significant pathogenic factor in allergic conjunctivitis. (J ALLERGY CLIN IMMUNOL 1995;95:663-7.).
AB - The pathogenesis of allergic conjunctivitis is largely conjectural. We investigated the possible involvement of respiratory syncytial virus (RSV), a ubiquitous respiratory pathogen, in the development of allergic conjunctivitis through immune mechanisms. A new technique of brush cytology was used to obtain conjunctival cells from 30 patients with allergic conjunctivitis and 20 control subjects. Samples were assayed for the presence of RSV sequences with the reverse-transcription polymerase chain reaction and the nested polymerase chain reaction. Specific primers and inner primers were synthesized on the basis of RNA sequences previously identified. RSV sequences were detected in 7 of 30 (23%) patient samples and 1 of 20 (5%) control samples. Our results proved that polymerase chain reaction could detect RSV sequences in conjunctival samples. RSV may be a significant pathogenic factor in allergic conjunctivitis. (J ALLERGY CLIN IMMUNOL 1995;95:663-7.).
KW - allergic conjunctivitis
KW - brush cytology
KW - nested PCR
KW - reverse-transcription polymerase chain reaction (RT-PCR)
UR - http://www.scopus.com/inward/record.url?scp=0028964391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028964391&partnerID=8YFLogxK
U2 - 10.1016/S0091-6749(95)70169-9
DO - 10.1016/S0091-6749(95)70169-9
M3 - Article
C2 - 7897147
AN - SCOPUS:0028964391
VL - 95
SP - 663
EP - 667
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 3
ER -