Retinal-specific ATP-binding cassette transporter (ABCR/ABCA4) is expressed at the choroid plexus in rat brain

Jiraganya Bhongsatiern, Sumio Ohtsuki, Masanori Tachikawa, Satoko Hori, Tetsuya Terasaki

研究成果: Article

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ATP-binding cassette (ABC) transporter A4 is a member of the ABC transporter subfamily A which has been reported to be exclusively expressed in the retina. In contrast, a previous report has suggested a possible relationship between ABCA4 and CNS function. The purpose of the present study was to investigate the localization of ABCA4 mRNA and protein in rat brain. In situ hybridization analysis revealed that ABCA4 mRNA was localized in the lateral ventricles. RT-PCR analysis detected ABCA4 mRNA in isolated rat choroid plexus and conditionally immortalized rat choroid plexus epithelial cells (TR-CSFB). Furthermore, ABCA4 protein was also detected in the isolated rat choroid plexus at about 250 KDa by western blot analysis, and its apparent molecular size was reduced by N-glycosidase F treatment. These results suggest that glycosylated ABCA4 protein is expressed in rat choroid plexus epithelial cells. ABCA4 may play a role in the function of the blood-cerebrospinal fluid barrier and affect CSF conditions.

元の言語English
ページ(範囲)1277-1280
ページ数4
ジャーナルJournal of Neurochemistry
92
発行部数5
DOI
出版物ステータスPublished - 2005 3 1

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ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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