Retinoic acid-induced gene expression of tissue transglutaminase via protein kinase c-dependent pathway in mouse peritoneal macrophages

Isao Ishii, Michio Ui

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Culture of mouse resident peritoneal macrophages with retinoic acid resulted in increased expression of the tissue transglutaminase gene as revealed by increases in the maximal velocity of the enzyme reaction in the cytosol and in the enzyme mRNA level. Protein kinase C-activating phorbol esters and okadaic acid, both of which were without effect on the enzyme induction by themselves, enhanced the retinoic acid-induced gene expression, which was in turn inhibited partially by pertussis toxin and totally by inhibitors of protein kinase C in either the presence or absence of phorbol esters. Retinoic acid was more effective in the "conditioned" medium, in which macrophages had been cultured for a time longer than 4 h, than in the "fresh" medium. The retinoic acid induction of transglutaminase was accompanied by increased phosphatidylinositol turnover and phosphatidic acid generation, which were efficiently suppressed by prior exposure of cells to pertussis toxin. It is likely that certain autocrine factor(s) liberated during culture of macrophages may afford conditions favorable for retinoic acid-induced gene expression, presumably via pertussis toxin-sensitive G protein-mediated phosphoinositide metabolism leading to activation of protein kinase C.

本文言語English
ページ(範囲)1197-1202
ページ数6
ジャーナルJournal of biochemistry
115
6
DOI
出版ステータスPublished - 1994 6

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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