Retrovirus-mediated gene transfer and expression cloning: Powerful tools in functional genomics

Toshio Kitamura, Yuko Koshino, Fumi Shibata, Toshihiko Oki, Hideaki Nakajima, Tetsuya Nosaka, Hidetoshi Kumagai

研究成果: Review article査読

576 被引用数 (Scopus)

抄録

Most of the human genome has now been sequenced and about 30,000 potential open reading frames have been identified, indicating that we use these 30,000 genes to functionally organize our biologic activities. However, functions of many genes are still unknown despite intensive efforts using bioinformatics as well as transgenic and knockout mice. Retrovirus-mediated gene transfer is a powerful tool that can be used to understand gene functions. We have developed a variety of retrovirus vectors and efficient packaging cell lines that have facilitated the development of efficient functional expression cloning methods. In this review, we describe retrovirus-mediated strategies used for investigation of gene functions and function-based screening strategies.

本文言語English
ページ(範囲)1007-1014
ページ数8
ジャーナルExperimental Hematology
31
11
DOI
出版ステータスPublished - 2003 11

ASJC Scopus subject areas

  • 分子生物学
  • 血液学
  • 遺伝学
  • 細胞生物学
  • 癌研究

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