Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain

Masanobu Yamada, Keiji Shimizu, Yasuyoshi Miyao, Toru Hayakawa, Kazuhiro Ikenaka, Kensuke Nakahira, Kazunori Nakajima, Tetsushi Kagawa, Katsuhiko Mikoshiba

研究成果: Article査読

17 被引用数 (Scopus)

抄録

A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coliβ‐galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV‐M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of β‐galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus‐mediated gene therapy for meningeal dissemination of malignant glioma.

本文言語English
ページ(範囲)1244-1247
ページ数4
ジャーナルJapanese Journal of Cancer Research
83
12
DOI
出版ステータスPublished - 1992 12
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

フィンガープリント

「Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル