RGD targeting of human ferritin iron oxide nanoparticles enhances in vivo MRI of vascular inflammation and angiogenesis in experimental carotid disease and abdominal aortic aneurysm

Toshiro Kitagawa, Hisanori Kosuge, Masaki Uchida, Yasunori Iida, Ronald L. Dalman, Trevor Douglas, Michael V. McConnell

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Purpose: To evaluate Arg-Gly-Asp (RGD)-conjugated human ferritin (HFn) iron oxide nanoparticles for in vivo magnetic resonance imaging (MRI) of vascular inflammation and angiogenesis in experimental carotid disease and abdominal aortic aneurysm (AAA). Materials and Methods: HFn was genetically engineered to express the RGD peptide and Fe3O4 nanoparticles were chemically synthesized inside the engineered HFn (RGD-HFn). Macrophage-rich left carotid lesions were induced by ligation in FVB mice made hyperlipidemic and diabetic (n = 14), with the contralateral right carotid serving as control. Murine AAAs were created by continuous angiotensin II infusion in ApoE-deficient mice (n = 12), while control mice underwent saline infusion (n = 8). All mice were imaged before and after intravenous injection with either RGD-HFn-Fe3O4 or HFn-Fe3O4 using a gradient-echo sequence on a whole-body 3T clinical scanner, followed by histological analysis. The nanoparticle accumulation was assessed by the extent of T* 2-induced carotid lumen reduction (% lumen loss) or aortic T* 2 -weighted signal intensity reduction (% SI [signal intensity] loss). Results: RGD-HFn-Fe3O4 was taken up more than HFn-Fe3O4 in both the ligated left carotid arteries (% lumen loss; 69 ± 9% vs. 36 ± 7%, P = 0.01) and AAAs (% SI loss; 47 ± 6% vs. 20 ± 5%, P = 0.01). The AAA % SI loss correlated positively with AAA size (r = 0.89, P < 0.001). Histology confirmed the greater accumulation and colocalization of RGD-HFn-Fe3O4 to both vascular macrophages and endothelial cells. Conclusion: RGD-HFn-Fe3O4 enhances in vivo MRI by targeting both vascular inflammation and angiogenesis, and provides a promising translatable MRI approach to detect high-risk atherosclerotic and aneurysmal vascular diseases. Level of Evidence: 1. J. Magn. Reson. Imaging 2017;45:1144–1153.

本文言語English
ページ(範囲)1144-1153
ページ数10
ジャーナルJournal of Magnetic Resonance Imaging
45
4
DOI
出版ステータスPublished - 2017 4 1

ASJC Scopus subject areas

  • 放射線学、核医学およびイメージング

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