RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans

Kumiko Oishi, Hideyuki Okano, Hitoshi Sawa

研究成果: Article査読

20 被引用数 (Scopus)

抄録

For proper chromosome segregation, the sister kinetochores must attach to microtubules extending from the opposite spindle poles. Any errors in microtubule attachment can induce aneuploidy. In this study, we identify a novel conserved Caenorhabditis elegans micro tubule-associated protein, regulator of microtubule dynamics 1 (RMD-1), that localizes to spindle microtubules and spindle poles. Depletion of RMD-1 induces severe defects in chromosome segregation, probably through merotelic attachments between microtubules and chromosomes. Although rmd-1 embryos also have a mild defect in microtubule growth, we find that mutants of the microtubule growth regulator XMAP215/ZYG-9 show much weaker segregation defects. This suggests that the microtubule growth defect in rmd-1 embryos does not cause abnormal chromosome segregation. We also see that RMD-1 interacts with aurora B in vitro. Our results suggest that RMD-1 functions in chromosome segregation in C. elegans embryos, possibly through the aurora B-mediated pathway. Human homologues of RMD-1 could also bind microtubules, which would suggest a function for these proteins in chromosome segregation during mitosis in other organisms as well.

本文言語English
ページ(範囲)1149-1162
ページ数14
ジャーナルJournal of Cell Biology
179
6
DOI
出版ステータスPublished - 2007 12月 17

ASJC Scopus subject areas

  • 細胞生物学

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