RNA and protein expression of the murine autoimmune regulator gene (Aire) in normal, RelB-deficient and in NOD mouse

Maarit Heino, Pärt Peterson, Niko Sillanpää, Sandrine Guérin, Li Wu, Graham Anderson, Hamish S. Scott, Stylianos E. Antonarakis, Jun Kudoh, Nobuyoshi Shimizu, Eric J. Jenkinson, Philippe Naquet, Kai J.E. Krohn

研究成果: Article査読

153 被引用数 (Scopus)

抄録

Mutations in the putative transcription factor autoimmune regulator (AIRE) gene are responsible for autoimmune polyendocrinopathy-candidiosis-ectodermal dystrophy (APECED; OMIM240300), a monogenic recessively inherited disease characterized by destructive autoimmune diseases of the endocrine organs, chronic candidiosis of mucous membranes and ectodermal dystrophies. In this study the expression of murine homolog for AIRE protein, Aire, was detected in a fraction of thymic medullary epithelial cells. Subcellularly, in thymus the protein appears as concentrated into nuclear dot-like structures, whereas in transfected cells the protein is also bound along a cytosolic fibrillar network. By RT-PCR Aire mRNA was detected in thymus, lymph node, spleen and testis although the second round PCR amplified Aire specific band from most mouse tissues analyzed. Furthermore, the Aire mRNA was detected in dendritic cell (DC) populations isolated from thymus and spleen, representing both myeloid- and lymphoid-related lineages of DC. We also demonstrate that the Aire protein is absent in the thymus of RelB-deficient mouse and in NOD thymus most of the Aire positive cells showed an abnormal morphology. These results suggest that the Aire protein is associated with the normal development and/or action of a subset of thymic medullary stromal cells involved in tolerance induction.

本文言語English
ページ(範囲)1884-1893
ページ数10
ジャーナルEuropean Journal of Immunology
30
7
DOI
出版ステータスPublished - 2000

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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