RNAi and overexpression of genes in ovarian somatic cells

Kuniaki Saito

研究成果: Chapter

抄録

Emerging evidence indicates that PIWI proteins, in collaboration with PIWI-interacting RNAs (piRNAs), play a critical role in retrotransposon silencing in Drosophila gonadal somatic and germ-line cells. The recent establishment of female germ-line stem cells/ovarian somatic sheet and its derivative cell line, ovarian somatic cells (OSCs), allows researchers to study the molecular functions of several protein factors involved in the primary piRNA pathway in Drosophila. Although transgene expression is difficult to achieve in gonad-derived cell lines, transfection of both expression vectors and knockdown reagents is highly effective in OSCs. Here, I focus on techniques that knockdown or overexpress genes of interest in OSCs.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
ページ25-33
ページ数9
1093
DOI
出版ステータスPublished - 2014

出版物シリーズ

名前Methods in Molecular Biology
1093
ISSN(印刷版)10643745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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