RNAi and overexpression of genes in ovarian somatic cells

Kuniaki Saito

研究成果: Chapter

5 被引用数 (Scopus)

抄録

Emerging evidence indicates that PIWI proteins, in collaboration with PIWI-interacting RNAs (piRNAs), play a critical role in retrotransposon silencing in Drosophila gonadal somatic and germ-line cells. The recent establishment of female germ-line stem cells/ovarian somatic sheet and its derivative cell line, ovarian somatic cells (OSCs), allows researchers to study the molecular functions of several protein factors involved in the primary piRNA pathway in Drosophila. Although transgene expression is difficult to achieve in gonad-derived cell lines, transfection of both expression vectors and knockdown reagents is highly effective in OSCs. Here, I focus on techniques that knockdown or overexpress genes of interest in OSCs.

元の言語English
ホスト出版物のタイトルPIWI-Interacting RNAs
ホスト出版物のサブタイトルMethods and Protocols
出版者Humana Press Inc.
ページ25-33
ページ数9
ISBN(印刷物)9781627036931
DOI
出版物ステータスPublished - 2014

出版物シリーズ

名前Methods in Molecular Biology
1093
ISSN(印刷物)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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