The present study was undertaken to investigate the expression and function of β1 integrins in human endometrium and decidua. Fluorescence-activated flow cytometry demonstrated the greater expression of the β1, α1, α2, and as subunits of the β1 integrin family in cultured stromal cells from the midsecretory phase than in those of the early proliferative phase. The addition of estradiol (E2) and progesterone (P) to cultured stromal cells in the early proliferative phase increased the expression of β1 integrins in vitro. The immunohistochemical distribution of β1 integrins demonstrated predominantly glandular epithelial staining in the proliferative phase, and stromal and glandular staining in the midsecretory phase. flow cytometry also demonstrated the expression of β1, α1, α2, α3, α5, and α6 subunits of β1 integrin family in cultured decidual cells. Immunohistochemistry confirmed the β1 integrin cell surface phenotypes in cultured decidual cells observed by flow cytometry. In the subsequent experiment, the effects of antibodies against specific β1 integrin heterodimers on mouse embryo attachment and spreading were tested to identify the role of β1 integrins in early implantation. We developed assays for the attachment of mouse embryos and for trophoblastic spreading on cultured human decidual cells. The addition of antibodies directed against β1 and α integrin subunits to cultured decidual cells did not affect the rates of hatching or attachment of the blastocysts, whereas the outgrowth of embryos on the decidual cells was inhibited by their antibodies in a dose-dependent manner. Thus, β1 integrin in human endometrium and decidua may be important in mediating the organization of extracellular matrix proteins derived from embryos during the early stage of implantation.
|出版ステータス||Published - 1998 7 1|
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