Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells

M. Fujita, R. Takahashi, P. Liang, Hideyuki Saya, F. Ashoori, M. Tachi, S. Kitazawa, S. Maeda

研究成果: Article

7 引用 (Scopus)

抄録

An alternatively splicing of the rat erythropoietin receptor (EpoR) gene was identified in normal and erythroleukemia cells by the reverse transcription PCR method. Insertion of a 105 bp fragment at the region corresponding to the extracellular domain of rat EpoR was found. The insert sequence, which encodes additional 21 amino acids, is similar to that previously found in the mouse EpoR gene, however, has an additional 27 bp direct repeat. Due to the presence of a stop codon in the insert, the alternative transcript is translated to a truncated and soluble form of EpoR which is preferentially expressed in liver, spleen, kidney, heart, and bone marrow cells as well as cultured erythroleukemia cells. These findings suggest that alternative splicing of the EpoR gene may play an important role in proliferation and differentiation of rat erythroid cells.

元の言語English
ページ(範囲)444-445
ページ数2
ジャーナルLeukemia
11
発行部数SUPPL. 3
出版物ステータスPublished - 1997
外部発表Yes

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Erythropoietin Receptors
Leukemia, Erythroblastic, Acute
Alternative Splicing
Genes
Erythroid Cells
Terminator Codon
Nucleic Acid Repetitive Sequences
Bone Marrow Cells
Reverse Transcription
Cultured Cells
Spleen
rat erythropoietin
Kidney
Amino Acids
Polymerase Chain Reaction
Liver

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

これを引用

Fujita, M., Takahashi, R., Liang, P., Saya, H., Ashoori, F., Tachi, M., ... Maeda, S. (1997). Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells. Leukemia, 11(SUPPL. 3), 444-445.

Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells. / Fujita, M.; Takahashi, R.; Liang, P.; Saya, Hideyuki; Ashoori, F.; Tachi, M.; Kitazawa, S.; Maeda, S.

:: Leukemia, 巻 11, 番号 SUPPL. 3, 1997, p. 444-445.

研究成果: Article

Fujita, M, Takahashi, R, Liang, P, Saya, H, Ashoori, F, Tachi, M, Kitazawa, S & Maeda, S 1997, 'Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells', Leukemia, 巻. 11, 番号 SUPPL. 3, pp. 444-445.
Fujita M, Takahashi R, Liang P, Saya H, Ashoori F, Tachi M その他. Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells. Leukemia. 1997;11(SUPPL. 3):444-445.
Fujita, M. ; Takahashi, R. ; Liang, P. ; Saya, Hideyuki ; Ashoori, F. ; Tachi, M. ; Kitazawa, S. ; Maeda, S. / Role of alternative splicing of the rat erythropoietin receptor gene in normal and erythroleukemia cells. :: Leukemia. 1997 ; 巻 11, 番号 SUPPL. 3. pp. 444-445.
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