Role of high mobility group box chromosomal protein 1 in ischemia-reperfusion injury in the rat small intestine

Masayuki Kojima, Minoru Tanabe, Masahiro Shinoda, Shingo Yamada, Taku Miyasho, Koichi Suda, Taizo Hibi, Hideaki Obara, Osamu Itano, Shigeyuki Kawachi, Masaki Kitajima, Ikuro Maruyama, Yuko Kitagawa

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Background: High mobility group box chromosomal protein 1 (HMGB1) has recently been shown to be an important late mediator of endotoxic shock and sepsis. The purpose of the present study was to investigate the role of HMGB1 in response to ischemia-reperfusion injury. Methods: Ischemia-reperfusion injury was induced in male Wistar rats by clamping the superior mesenteric artery for 60 min. Using this model, the serum concentrations and localization of HMGB1 were investigated. The histologic findings from reperfused intestines and the survival rates were compared between the anti-HMGB1 antibody treatment groups (group A treated with 6.0 mg/kg antibody and group B with 0.6 mg/kg antibody) and the control antibody treatment group (control group). Results: Serum HMGB1 concentrations increased early after reperfusion and peaked at 3 h. Immunohistochemistry for HMGB1 revealed a high degree of positive staining in the epithelial cells of the damaged villi. Anti-HMGB1 antibody treatment significantly reduced this damage (P < 0.05) and improved the 48-h survival rate (90% in group A versus 50% in the controls; P < 0.05). Conclusions: These results suggest that HMGB1 plays a key role in small intestinal ischemia-reperfusion injury.

本文言語English
ページ(範囲)466-471
ページ数6
ジャーナルJournal of Surgical Research
178
1
DOI
出版ステータスPublished - 2012 11 1

ASJC Scopus subject areas

  • Surgery

フィンガープリント 「Role of high mobility group box chromosomal protein 1 in ischemia-reperfusion injury in the rat small intestine」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル