Role of interleukin-6 in elastase-induced lung inflammatory changes in mice

Sadatomo Tasaka, Ken Ichiro Inoue, Keisuke Miyamoto, Yasushi Nakano, Hirofumi Kamata, Hiromi Shinoda, Naoki Hasegawa, Taku Miyasho, Masahiko Satoh, Hirohisa Takano, Akitoshi Ishizaka

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Interleukin-6 (IL-6) is known to be involved in the pathogenesis of various inflammatory diseases, but its role in the development of pulmonary emphysema remains unclear. Wild-type (WT) and IL-6deficient mice received either phosphate-buffered saline (PBS) or porcine pancreatic elastase (PPE) intratracheally. The development of emphysema was determined by measuring the mean linear intercept (Lm). The lung specimens were also subjected to immunohistochemistry for single-stranded DNA to detect apoptotic cells. Lung mechanics and airway responsiveness to inhaled methacholine were analyzed. Bronchoalveolar lavage (BAL) fluid was subjected to evaluation of inflammatory cell accumulation and cytokine measurement. PPE treatment caused significant increases in Lm and lung compliance, which was attenuated by IL-6 deficiency. The increases in apoptotic cells in the lung were attenuated in IL-6 null mice. Airway responsiveness was not affected by PPE challenge or IL-6 deficiency. Intratracheal PPE increased the cell counts in BAL fluid throughout the observation, which was suppressed in IL-6 null mice. In BAL fluid, PPE-induced increases in the levels of macrophage inflammatory protein (MIP)-1α and eotaxin were mitigated by IL-6 deficiency. PPE-induced up-regulation of matrix metalloproteinase (MMP)-12 in the lung was attenuated by IL-6 deficiency. These results indicate that IL-6 may play an important role in the development of elastase-induced lung inflammatory changes.

本文言語English
ページ(範囲)362-372
ページ数11
ジャーナルExperimental Lung Research
36
6
DOI
出版ステータスPublished - 2010 7

ASJC Scopus subject areas

  • 分子生物学
  • 呼吸器内科
  • 臨床生化学

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