Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila

Kuniaki Saito, Hirotsugu Ishizu, Miharu Komai, Hazuki Kotani, Yoshinori Kawamura, Kazumichi M. Nishida, Haruhiko Siomi, Mikiko C. Siomi

研究成果: Article

197 引用 (Scopus)

抄録

PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.

元の言語English
ページ(範囲)2493-2498
ページ数6
ジャーナルGenes and Development
24
発行部数22
DOI
出版物ステータスPublished - 2010 11 15

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Small Interfering RNA
Drosophila
RNA
Carisoprodol
Genome

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

これを引用

Saito, K., Ishizu, H., Komai, M., Kotani, H., Kawamura, Y., Nishida, K. M., ... Siomi, M. C. (2010). Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. Genes and Development, 24(22), 2493-2498. https://doi.org/10.1101/gad.1989510

Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. / Saito, Kuniaki; Ishizu, Hirotsugu; Komai, Miharu; Kotani, Hazuki; Kawamura, Yoshinori; Nishida, Kazumichi M.; Siomi, Haruhiko; Siomi, Mikiko C.

:: Genes and Development, 巻 24, 番号 22, 15.11.2010, p. 2493-2498.

研究成果: Article

Saito, K, Ishizu, H, Komai, M, Kotani, H, Kawamura, Y, Nishida, KM, Siomi, H & Siomi, MC 2010, 'Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila', Genes and Development, 巻. 24, 番号 22, pp. 2493-2498. https://doi.org/10.1101/gad.1989510
Saito, Kuniaki ; Ishizu, Hirotsugu ; Komai, Miharu ; Kotani, Hazuki ; Kawamura, Yoshinori ; Nishida, Kazumichi M. ; Siomi, Haruhiko ; Siomi, Mikiko C. / Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. :: Genes and Development. 2010 ; 巻 24, 番号 22. pp. 2493-2498.
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AU - Kotani, Hazuki

AU - Kawamura, Yoshinori

AU - Nishida, Kazumichi M.

AU - Siomi, Haruhiko

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