Roles of ES cell-derived gliogenic neural stem/progenitor cells in functional recovery after spinal cord injury

Gentaro Kumagai, Yohei Okada, Junichi Yamane, Narihito Nagoshi, Kazuya Kitamura, Masahiko Mukaino, Osahiko Tsuji, Kanehiro Fujiyoshi, Hiroyuki Katoh, Seiji Okada, Shinsuke Shibata, Yumi Matsuzaki, Satoshi Toh, Yoshiaki Toyama, Masaya Nakamura, Hideyuki Okano

研究成果: Article

86 引用 (Scopus)

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Transplantation of neural stem/progenitor cells (NS/PCs) following the sub-acute phase of spinal cord injury (SCI) has been shown to promote functional recovery in rodent models. However, the types of cells most effective for treating SCI have not been clarified. Taking advantage of our recently established neurosphere-based culture system of ES cell-derived NS/PCs, in which primary neurospheres (PNS) and passaged secondary neurospheres (SNS) exhibit neurogenic and gliogenic potentials, respectively, here we examined the distinct effects of transplanting neurogenic and gliogenic NS/PCs on the functional recovery of a mouse model of SCI. ES cell-derived PNS and SNS transplanted 9 days after contusive injury at the Th10 level exhibited neurogenic and gliogenic differentiation tendencies, respectively, similar to those seen in vitro. Interestingly, transplantation of the gliogenic SNS, but not the neurogenic PNS, promoted axonal growth, remyelination, and angiogenesis, and resulted in significant locomotor functional recovery after SCI. These findings suggest that gliogenic NS/PCs are effective for promoting the recovery from SCI, and provide essential insight into the mechanisms through which cellular transplantation leads to functional improvement after SCI.

元の言語English
記事番号e7706
ジャーナルPloS one
4
発行部数11
DOI
出版物ステータスPublished - 2009 11 6

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Kumagai, G., Okada, Y., Yamane, J., Nagoshi, N., Kitamura, K., Mukaino, M., Tsuji, O., Fujiyoshi, K., Katoh, H., Okada, S., Shibata, S., Matsuzaki, Y., Toh, S., Toyama, Y., Nakamura, M., & Okano, H. (2009). Roles of ES cell-derived gliogenic neural stem/progenitor cells in functional recovery after spinal cord injury. PloS one, 4(11), [e7706]. https://doi.org/10.1371/journal.pone.0007706