Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1

Y. Morita, N. Kimura, T. Mima, T. Mizushima, T. Tsuchiya

研究成果: Article

51 引用 (Scopus)

抄録

To envisage the roles of MexXY- and MexAB-multidrug efflux pumps in the intrinsic multidrug resistance of wild-type strain Pseudomonas aeruginosa PAO1, we constructed mutants lacking either individual or both efflux pumps. A mutant lacking MexXY showed increased susceptibility to aminoglycosides, erythromycin, and tetracycline, but not to β-lactams, chloramphenicol, or quinolones. A mutant lacking MexAB showed increased susceptibility to β-lactams, chloramphenicol, and nalidixic acid, but not to aminoglycosides, erythromycin, tetracycline, or fluoroquinolones. A mutant lacking both MexXY and MexAB showed an increased susceptibility to all antimicrobial agents tested compared with the wild type. Very similar results were obtained with a mutant lacking MexAB-OprM and a mutant lacking both MexXY and MexAB-OprM. Thus it is clear that OprM is essential not only for the function of MexAB, but also for the function of MexXY. Furthermore, we found that each pump compensated to some extent for the lack of another pump with respect to the common substrates (tetracycline, quinolones, and cefpirome). The introduction of a plasmid carrying the mexXY genes into P. aeruginosa PAO1 cells increased the resistance to fluoroquinolones. This suggests that the mexXY genes could be involved in acquired resistance to fluoroquinolones in P. aeruginosa PAO1.

元の言語English
ページ(範囲)27-32
ページ数6
ジャーナルJournal of General and Applied Microbiology
47
発行部数1
出版物ステータスPublished - 2001
外部発表Yes

Fingerprint

Fluoroquinolones
Multiple Drug Resistance
Tetracycline
Pseudomonas aeruginosa
Lactams
cefpirome
Quinolones
Aminoglycosides
Chloramphenicol
Erythromycin
Nalidixic Acid
Anti-Infective Agents
Genes
Plasmids

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

これを引用

Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1. / Morita, Y.; Kimura, N.; Mima, T.; Mizushima, T.; Tsuchiya, T.

:: Journal of General and Applied Microbiology, 巻 47, 番号 1, 2001, p. 27-32.

研究成果: Article

Morita, Y. ; Kimura, N. ; Mima, T. ; Mizushima, T. ; Tsuchiya, T. / Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1. :: Journal of General and Applied Microbiology. 2001 ; 巻 47, 番号 1. pp. 27-32.
@article{bc3fe6461efe42cdaa95b45d84e3f86e,
title = "Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1",
abstract = "To envisage the roles of MexXY- and MexAB-multidrug efflux pumps in the intrinsic multidrug resistance of wild-type strain Pseudomonas aeruginosa PAO1, we constructed mutants lacking either individual or both efflux pumps. A mutant lacking MexXY showed increased susceptibility to aminoglycosides, erythromycin, and tetracycline, but not to β-lactams, chloramphenicol, or quinolones. A mutant lacking MexAB showed increased susceptibility to β-lactams, chloramphenicol, and nalidixic acid, but not to aminoglycosides, erythromycin, tetracycline, or fluoroquinolones. A mutant lacking both MexXY and MexAB showed an increased susceptibility to all antimicrobial agents tested compared with the wild type. Very similar results were obtained with a mutant lacking MexAB-OprM and a mutant lacking both MexXY and MexAB-OprM. Thus it is clear that OprM is essential not only for the function of MexAB, but also for the function of MexXY. Furthermore, we found that each pump compensated to some extent for the lack of another pump with respect to the common substrates (tetracycline, quinolones, and cefpirome). The introduction of a plasmid carrying the mexXY genes into P. aeruginosa PAO1 cells increased the resistance to fluoroquinolones. This suggests that the mexXY genes could be involved in acquired resistance to fluoroquinolones in P. aeruginosa PAO1.",
keywords = "Instrinsic drug resistance, MexAB, MexXY, Multidrug efflux pump, Pseudomonas aeruginosa",
author = "Y. Morita and N. Kimura and T. Mima and T. Mizushima and T. Tsuchiya",
year = "2001",
language = "English",
volume = "47",
pages = "27--32",
journal = "Journal of General and Applied Microbiology",
issn = "0022-1260",
publisher = "Microbiology Research Foundation",
number = "1",

}

TY - JOUR

T1 - Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1

AU - Morita, Y.

AU - Kimura, N.

AU - Mima, T.

AU - Mizushima, T.

AU - Tsuchiya, T.

PY - 2001

Y1 - 2001

N2 - To envisage the roles of MexXY- and MexAB-multidrug efflux pumps in the intrinsic multidrug resistance of wild-type strain Pseudomonas aeruginosa PAO1, we constructed mutants lacking either individual or both efflux pumps. A mutant lacking MexXY showed increased susceptibility to aminoglycosides, erythromycin, and tetracycline, but not to β-lactams, chloramphenicol, or quinolones. A mutant lacking MexAB showed increased susceptibility to β-lactams, chloramphenicol, and nalidixic acid, but not to aminoglycosides, erythromycin, tetracycline, or fluoroquinolones. A mutant lacking both MexXY and MexAB showed an increased susceptibility to all antimicrobial agents tested compared with the wild type. Very similar results were obtained with a mutant lacking MexAB-OprM and a mutant lacking both MexXY and MexAB-OprM. Thus it is clear that OprM is essential not only for the function of MexAB, but also for the function of MexXY. Furthermore, we found that each pump compensated to some extent for the lack of another pump with respect to the common substrates (tetracycline, quinolones, and cefpirome). The introduction of a plasmid carrying the mexXY genes into P. aeruginosa PAO1 cells increased the resistance to fluoroquinolones. This suggests that the mexXY genes could be involved in acquired resistance to fluoroquinolones in P. aeruginosa PAO1.

AB - To envisage the roles of MexXY- and MexAB-multidrug efflux pumps in the intrinsic multidrug resistance of wild-type strain Pseudomonas aeruginosa PAO1, we constructed mutants lacking either individual or both efflux pumps. A mutant lacking MexXY showed increased susceptibility to aminoglycosides, erythromycin, and tetracycline, but not to β-lactams, chloramphenicol, or quinolones. A mutant lacking MexAB showed increased susceptibility to β-lactams, chloramphenicol, and nalidixic acid, but not to aminoglycosides, erythromycin, tetracycline, or fluoroquinolones. A mutant lacking both MexXY and MexAB showed an increased susceptibility to all antimicrobial agents tested compared with the wild type. Very similar results were obtained with a mutant lacking MexAB-OprM and a mutant lacking both MexXY and MexAB-OprM. Thus it is clear that OprM is essential not only for the function of MexAB, but also for the function of MexXY. Furthermore, we found that each pump compensated to some extent for the lack of another pump with respect to the common substrates (tetracycline, quinolones, and cefpirome). The introduction of a plasmid carrying the mexXY genes into P. aeruginosa PAO1 cells increased the resistance to fluoroquinolones. This suggests that the mexXY genes could be involved in acquired resistance to fluoroquinolones in P. aeruginosa PAO1.

KW - Instrinsic drug resistance

KW - MexAB

KW - MexXY

KW - Multidrug efflux pump

KW - Pseudomonas aeruginosa

UR - http://www.scopus.com/inward/record.url?scp=0035022408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035022408&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0035022408

VL - 47

SP - 27

EP - 32

JO - Journal of General and Applied Microbiology

JF - Journal of General and Applied Microbiology

SN - 0022-1260

IS - 1

ER -