Selection of ganglioside GM1-binding peptides by using a phage library

Teruhiko Matsubara, Dai Ishikawa, Takao Taki, Yoshio Okahata, Toshinori Sato

研究成果: Article査読

55 被引用数 (Scopus)

抄録

Ganglioside Galβ1→3GalNAcβ1→4(NeuAcα2→3)Galβ1→4Glcβ1→1'Cer (GM1)-binding peptides were obtained from a phage-displayed pentadecapeptide library by an affinity selection. The selection processes were in situ-monitored by a quartz-crystal microbalance method, on which a ganglioside GM1 monolayer was transferred. After five rounds of biopanning, the DNA sequencing of 18 selected phages showed that only three individual clones were selected. The peptide sequences of the random region were found to be DFRRLPGAFWQLRQP, GWWYKGRARPVSAVA and VWRLLAPPFSNRLLP. Binding constants of these phage clones to the GM1 monolayer were 1010 M-1. Three synthetic pentadecapeptides inhibited the binding of cholera toxin B subunit to the GM1 monolayer with an IC50 of 24, 13 and 1.0 μM, respectively. These peptides will be useful for searching functional roles of ganglioside GM1. Copyright (C) 1999 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)253-256
ページ数4
ジャーナルFEBS Letters
456
2
DOI
出版ステータスPublished - 1999 8月 6
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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