Selective isolation of DNA or RNA using single-stranded DNA affinity latex particles

Takeshi Imai, Yoshiteru Sumi, Mamoru Hatakeyama, Keiji Fujimoto, Haruma Kawaguchi, Nobuaki Hayashida, Kazuo Shiozaki, Kunihiko Terada, Hiroaki Yajima, Hiroshi Handa

研究成果: Article査読

29 被引用数 (Scopus)

抄録

We developed latex particles with a diameter of about 0.22 μm on which single-stranded (ss) DNA was covalently coupled to select or enrich its complementary DNA or mRNA. DNA was first covalently coupled to the latex particles in the double-stranded (ds) form with both blunt and protruding ends. More than 80% of the dsDNA was coupled through the ssDNA stretch at its protruding end. The presence of NaCl in the immobilization reaction severely inhibited DNA from coupling to the particles. The particles were then treated with alkali or heated to denature the dsDNA and sedimented by a brief centrifugation to yield ssDNA immobilized particles. They allowed the selective and efficient isolation of a desired RNA from total cellular RNA.

本文言語English
ページ(範囲)245-249
ページ数5
ジャーナルJournal of Colloid And Interface Science
177
1
DOI
出版ステータスPublished - 1996 1 15

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 表面、皮膜および薄膜
  • コロイド化学および表面化学

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