Selective killing of fibroblast-like cells in cultures of mouse heart cells by treatment with a Ca ionophore, A23187

Hiroyuki Kaneko, Kiyota Goshima

研究成果: Article

9 引用 (Scopus)

抄録

Myocardial cells uncontaminated by fibroblast-like cells could be obtained by treating mouse heart cell cultures with a Ca ionophore, A23187. When trypsin-dissociated mouse heart cells were cultivated for 1 day or more on Petri dishes, both myocardial and fibroblast-like cells were detected on the dish under a phase contrast microscope. However, when the cultured heart cells were treated for a short period (e.g. 10 min) at 25 °C with A23187, only fibroblast-like cells exhibited morphological degeneration, such as nuclear pycnosis, cytoplasmic condensation and bleb- and balloon-formation. The myocardial cells maintained their normal morphology during the treatment; transient irregular beating was observed, but disappeared after removal of A23187. Thus treatment of heart cell cultures with A23187 can yield a large amount of myocardial cells without contamination by fibroblast-like cells. The possible mechanism for this selective effect is discussed.

元の言語English
ページ(範囲)407-416
ページ数10
ジャーナルExperimental Cell Research
142
発行部数2
DOI
出版物ステータスPublished - 1982
外部発表Yes

Fingerprint

Ionophores
Calcimycin
Cell Culture Techniques
Fibroblasts
Blister
Trypsin
Cultured Cells

ASJC Scopus subject areas

  • Cell Biology

これを引用

@article{6b08844140304336bf3874f48ba7762f,
title = "Selective killing of fibroblast-like cells in cultures of mouse heart cells by treatment with a Ca ionophore, A23187",
abstract = "Myocardial cells uncontaminated by fibroblast-like cells could be obtained by treating mouse heart cell cultures with a Ca ionophore, A23187. When trypsin-dissociated mouse heart cells were cultivated for 1 day or more on Petri dishes, both myocardial and fibroblast-like cells were detected on the dish under a phase contrast microscope. However, when the cultured heart cells were treated for a short period (e.g. 10 min) at 25 °C with A23187, only fibroblast-like cells exhibited morphological degeneration, such as nuclear pycnosis, cytoplasmic condensation and bleb- and balloon-formation. The myocardial cells maintained their normal morphology during the treatment; transient irregular beating was observed, but disappeared after removal of A23187. Thus treatment of heart cell cultures with A23187 can yield a large amount of myocardial cells without contamination by fibroblast-like cells. The possible mechanism for this selective effect is discussed.",
author = "Hiroyuki Kaneko and Kiyota Goshima",
year = "1982",
doi = "10.1016/0014-4827(82)90382-2",
language = "English",
volume = "142",
pages = "407--416",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Selective killing of fibroblast-like cells in cultures of mouse heart cells by treatment with a Ca ionophore, A23187

AU - Kaneko, Hiroyuki

AU - Goshima, Kiyota

PY - 1982

Y1 - 1982

N2 - Myocardial cells uncontaminated by fibroblast-like cells could be obtained by treating mouse heart cell cultures with a Ca ionophore, A23187. When trypsin-dissociated mouse heart cells were cultivated for 1 day or more on Petri dishes, both myocardial and fibroblast-like cells were detected on the dish under a phase contrast microscope. However, when the cultured heart cells were treated for a short period (e.g. 10 min) at 25 °C with A23187, only fibroblast-like cells exhibited morphological degeneration, such as nuclear pycnosis, cytoplasmic condensation and bleb- and balloon-formation. The myocardial cells maintained their normal morphology during the treatment; transient irregular beating was observed, but disappeared after removal of A23187. Thus treatment of heart cell cultures with A23187 can yield a large amount of myocardial cells without contamination by fibroblast-like cells. The possible mechanism for this selective effect is discussed.

AB - Myocardial cells uncontaminated by fibroblast-like cells could be obtained by treating mouse heart cell cultures with a Ca ionophore, A23187. When trypsin-dissociated mouse heart cells were cultivated for 1 day or more on Petri dishes, both myocardial and fibroblast-like cells were detected on the dish under a phase contrast microscope. However, when the cultured heart cells were treated for a short period (e.g. 10 min) at 25 °C with A23187, only fibroblast-like cells exhibited morphological degeneration, such as nuclear pycnosis, cytoplasmic condensation and bleb- and balloon-formation. The myocardial cells maintained their normal morphology during the treatment; transient irregular beating was observed, but disappeared after removal of A23187. Thus treatment of heart cell cultures with A23187 can yield a large amount of myocardial cells without contamination by fibroblast-like cells. The possible mechanism for this selective effect is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0020352452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020352452&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(82)90382-2

DO - 10.1016/0014-4827(82)90382-2

M3 - Article

C2 - 6816622

AN - SCOPUS:0020352452

VL - 142

SP - 407

EP - 416

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -