Sentan: A novel specific component of the apical structure of vertebrate motile cilia

Akiharu Kubo, Akiko Yuba-Kubo, Sachiko Tsukita, Shoichiro Tsukita, Masayuki Amagai

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Human respiratory and oviductal cilia have specific apical structures characterized by a narrowed distal portion and a ciliary crown. These structures are conserved among vertebrates that have air respiration systems; however, the molecular components of these structures have not been defined, and their functions are unknown. To identify the molecular component(s) of the cilia apical structure, we screened EST libraries to identify gene(s) that are exclusively expressed in ciliated tissues, are transcriptionally up-regulated during in vitro ciliogenesis, and are not expressed in testis (because sperm flagella have no such apical structures). One of the identified gene products, named sentan, was localized to the distal tip region of motile cilia. Using anti-sentan polyclonal antibodies and electron microscopy, sentan was shown to localize exclusively to the bridging structure between the cell membrane and peripheral singlet microtubules, which specifically exists in the narrowed distal portion of cilia. Exogenously expressed sentan showed affinity for the membrane protrusions, and a protein-lipid binding assay revealed that sentan bound to phosphatidylserine. These findings suggest that sentan is the first molecular component of the ciliary tip to bridge the cell membrane and peripheral singlet microtubules, making the distal portion of the cilia narrow and stiff to allow for better airway clearance or ovum transport.

本文言語English
ページ(範囲)5338-5346
ページ数9
ジャーナルMolecular biology of the cell
19
12
DOI
出版ステータスPublished - 2008 12月

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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