Separation of μ-opioid receptor desensitization and internalization: Endogenous receptors in primary neuronal cultures

Seksiri Arttamangkul, Maria Torrecilla, Kazuto Kobayashi, Hideyuki Okano, John T. Williams

研究成果: Article査読

67 被引用数 (Scopus)

抄録

A close relationship between desensitization and internalization of μ-opioid receptors (MORs) has been proposed based on differential actions of series of agonists. The role that these two processes have in the development of tolerance and dependence to opioids has been a controversial subject that has been studied in a variety of model systems. Here, we examine desensitization and internalization of endogenous MORs simultaneously in primary cultures of locus ceruleus neurons using fluorescently tagged peptide agonists. With the use of two fluorescent opioid peptides, dermorphin-Bodipy Texas Red and dermorphin-Alexa594 (Derm-A594), desensitization was measured electrophysiologically and trafficking was followed by the accumulation of intracellular fluorescent puncta. Blocking endocytosis with concanavalin A eliminated the accumulation of fluorescent puncta but desensitization induced by Derm-A594 was unaffected. Likewise, after treatment with concanavalin A, there was no change in either desensitization or recovery from desensitization induced by [Met]5 enkephalin. The results demonstrate that desensitization and the recovery from desensitization are not dependent on receptor internalization and suggest that the activity of endogenous MORs in primary neurons can be modulated at the level of the plasma membrane.

本文言語English
ページ(範囲)4118-4125
ページ数8
ジャーナルJournal of Neuroscience
26
15
DOI
出版ステータスPublished - 2006

ASJC Scopus subject areas

  • 神経科学(全般)

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